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Tg(Thy1-Clomeleon)1Gjau
Transgene Detail
Summary
Symbol: Tg(Thy1-Clomeleon)1Gjau
Name: transgene insertion 1, George Augustine
MGI ID: MGI:4847924
Synonyms: Tg(Thy1-CFP,Topaz)CLM1Gjau, Tg(Thy1-CFP,YFP)CLM1Gjau
Transgene: Tg(Thy1-Clomeleon)1Gjau  Location: unknown  
Alliance: Tg(Thy1-Clomeleon)1Gjau page
Transgene
origin
Strain of Origin:  ICR
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA transgenic construct containing Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2) was injected into fertilized ICR mouse eggs. Founder line CLM1 was subsequently established. Transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and YFP (topaz) that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM1 is detected at high levels in the mitral cells of the olfactory bulb, layer 5 of the neocortex, hippocampal dentate gyrus and CA1 region, amygdala, cerebellar granule cells, and retinal bipolar cells. No expression is detected in cerebellar mossy fibers, Purkinje cells or nuclei. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. (J:166510)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:165251 Duebel J, et al., Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Neuron. 2006 Jan 5;49(1):81-94
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory