Tg(Thy1-Clomeleon)13Gjau
Transgene Detail

Summary

• Symbol: Tg(Thy1-Clomeleon)13Gjau
• Name: transgene insertion 13, George Augustine
• MGI ID: MGI:4847926
• Synonyms: Tg(Thy1-CFP,Topaz)CLM13Gjau, Tg(Thy1-CFP,YFP)CLM13Gjau
• Transgene: Tg(Thy1-Clomeleon)13Gjau Location: unknown
• Alliance: Tg(Thy1-Clomeleon)13Gjau page

Transgene origin

• Strain of Origin: ICR

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A transgenic construct containing Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2) was injected into fertilized ICR mouse eggs. Founder line CLM13 was subsequently established. Transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM13 is detected at high levels in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum. No expression is detected in the external plexi form layer, mitral cells or internal plexiform layer of the olfactory bulb, layer 1 of the neocortex, cerebellar granule or purkinje cells, or retinal biopolar and amacrine cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. (J:166512)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:165251 Duebel J, et al., Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Neuron. 2006 Jan 5;49(1):81-94
• All: 4 reference(s)