Col1a1^{tm1(tetO-Lin28a)Gqda}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Lin28a)Gqda}
• Name: collagen, type I, alpha 1; targeted mutation 1, George Q Daley
• MGI ID: MGI:4849370
• Synonyms: Lin28a Tg
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Lin28a)Gqda} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166568
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-Lin28a)Gqda} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lin28a (MGI:1890546)

• Col1a1^{tm1(tetO-Lin28a)Gqda} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, flag-tagged mouse cDNA, and an SV40 polyadenylation signal into the 3'UTR. Leaky ectopic expression in the absence of doxycycline induction was confirmed by quantitative RT-PCR and immunohistochemistry. (J:166568)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*))Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:166568 Zhu H, et al., Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet. 2010 Jul;42(7):626-30
• All: 11 reference(s)