Lin28a^tm1Egm
Targeted Allele Detail

Summary

• Symbol: Lin28a^tm1Egm
• Name: lin-28 homolog A; targeted mutation 1, Eric G Moss
• MGI ID: MGI:4849371
• Gene: Lin28a Location: Chr4:133730641-133746152 bp, - strand Genetic Position: Chr4, 66.5 cM
• Alliance: Lin28a^tm1Egm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166568
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. A floxed neomycin resistance (neo) cassette was inserted downstream of exon 2. Cre-mediated recombination removed exon 2 and the neo cassette. Western blot analysis confirmed the absence of protein expression in embryonic stem cell lysate. (J:196313)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Lin28a^tm1Egm/Lin28a^tm1Egm	involves: 129S/SvEv
hm2	Lin28a^tm1Egm/Lin28a^tm1Egm	involves: 129S/SvEv * C57BL/6NTac

Phenotypes:

Affected Systems	hm1	hm2
show or hide all annotated terms Sex:
behavior/neurological			√
decreased male mating frequency			√
cellular		√	√
decreased germ cell number		√	√
decreased oocyte number		√
oligozoospermia			√
decreased primordial germ cell number		√	√
endocrine/exocrine glands		√
decreased primordial ovarian follicle number		√
decreased ovary weight		√
growth/size/body	√	√	√
decreased birth weight	√
decreased body size		√	√
decreased body weight		√	√
homeostasis/metabolism			√
decreased circulating testosterone level			√
decreased circulating follicle stimulating hormone level			√
mortality/aging	√
postnatal lethality, complete penetrance	√
reproductive system		√	√
decreased germ cell number		√	√
decreased oocyte number		√
oligozoospermia			√
decreased primordial germ cell number		√	√
decreased primordial ovarian follicle number		√
decreased ovary weight		√
reduced female fertility		√
decreased litter size		√	√
reduced male fertility			√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lin28a Mutation: 76 strains or lines available

References

• Original: J:166568 Zhu H, et al., Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet. 2010 Jul;42(7):626-30
• All: 6 reference(s)