Tg(Tmem100-EGFP/cre/ERT2)30Amc
Transgene Detail

Summary

• Symbol: Tg(Tmem100-EGFP/cre/ERT2)30Amc
• Name: transgene insertion 30, Andrew P McMahon
• MGI ID: MGI:4849986
• Synonyms: Tmem100-GCE, Tmem^GCE
• Transgene: Tg(Tmem100-EGFP/cre/ERT2)30Amc Location: unknown
• Alliance: Tg(Tmem100-EGFP/cre/ERT2)30Amc page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Tmem100-EGFP/cre/ERT2)30Amc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tmem100 (MGI:1915138)

• Mutation details: A targeting construct containing the eGFPCreERT2 (GCE) cassette and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-183L17). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 30 was subsequently established. Native GFP expression is detectable in the urogenital system of E15.5 embryos. Transgenic cre expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts, and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. Possible cre recombinase activity is detected in small renal arteries. (J:159099)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tmem100 (mouse) Summary of all recombinase alleles driven by Tmem100. Comparative matrix view of recombinase activities Alliance mouse Tmem100 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 1 reference(s)