Tg(S100b-EGFP/cre/ERT2)22Amc
Transgene Detail

Summary

• Symbol: Tg(S100b-EGFP/cre/ERT2)22Amc
• Name: transgene insertion 22, Andrew P McMahon
• MGI ID: MGI:4849988
• Synonyms: S100b-GCE
• Transgene: Tg(S100b-EGFP/cre/ERT2)22Amc Location: unknown
• Alliance: Tg(S100b-EGFP/cre/ERT2)22Amc page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(S100b-EGFP/cre/ERT2)22Amc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	S100b (MGI:98217)

• Mutation details: A targeting construct containing the eGFPCreERT2 (GCE) cassette and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-213P2). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 22 was subsequently established. GFP fluorescence is not detectable by fluorescent microscope examination of whole embryos, bones or neural tube sagittal slices from embryos aged 15.5dpc. Tamoxifen induced cre recombinase activity is detected in a subset of the GFP immunoreactive-positive cells in bone. (J:159099)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	S100b (mouse) Summary of all recombinase alleles driven by S100b. Comparative matrix view of recombinase activities Alliance mouse S100b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 1 reference(s)