Evx2/Hoxd13^{tm5(Hoxd11/lacZ)Ddu}
Targeted Allele Detail

Summary

• Symbol: Evx2/Hoxd13^{tm5(Hoxd11/lacZ)Ddu}
• Name: homeobox D13; targeted mutation 5, Denis Duboule
• MGI ID: MGI:4850024
• Synonyms: TgH[d-11/lac/neo]Ge
• Gene: Hoxd13 Location: Chr2:74498654-74501943 bp, + strand Genetic Position: Chr2, 44.13 cM
• Alliance: Evx2/Hoxd13^{tm5(Hoxd11/lacZ)Ddu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33879
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The E. coli lacZ gene was inserted, with translational reading frames aligned, into the first exon of the mouse Hoxd11 gene in a construct containing the entire Hoxd11 transcription unit and its known cis-acting 5'and 3' regulatory sequences. This fusion gene, followed by a loxP-flanked PGK-neo selection cassette, was targeted to the intergenic region between the transcription initiation sites of Evx2 and Hoxd13, at the 5' extremity of the Hoxd complex. (J:33879)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxd13 Mutation: 24 strains or lines available

References

• Original: J:33879 van der Hoeven F, et al., Gene transpositions in the HoxD complex reveal a hierarchy of regulatory controls. Cell. 1996 Jun 28;85(7):1025-35
• All: 1 reference(s)