Evx2/Hoxd13^{tm5.1(Hoxd11/lacZ)Ddu}
Targeted Allele Detail

Summary

• Symbol: Evx2/Hoxd13^{tm5.1(Hoxd11/lacZ)Ddu}
• Name: homeobox D13; targeted mutation 5.1, Denis Duboule
• MGI ID: MGI:4850027
• Synonyms: Evx2Hoxd13^{Tg(hoxd11lac)Ddu}, HoxD^TgHd11lacNsi, TgH13(d11lac), TgH[d-11/lac/neo]Ge
• Gene: Hoxd13 Location: Chr2:74498654-74501943 bp, + strand Genetic Position: Chr2, 44.13 cM
• Alliance: Evx2/Hoxd13^{tm5.1(Hoxd11/lacZ)Ddu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33879
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The E. coli lacZ gene was inserted, with translational reading frames aligned, into the first exon of the mouse Hoxd11 gene in a construct containing the entire Hoxd11 transcription unit and its known cis-acting 5'and 3' regulatory sequences. A construct containing this fusion gene followed by an oppositely oriented loxP-flanked PGK-neo selection cassette was targeted to the intergenic region between the transcription initiation sites of Evx2 and Hoxd13, at the 5' extremity of the Hoxd complex, with Hoxd11/lacZ in the same transcriptional orientation as Hoxd13. The selection cassette was subsequently deleted by Cre recombinase-mediated recombination, leaving a single loxP site downstream/3' of the Hoxd/lacZ fusion gene. (J:33879)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxd13 Mutation: 24 strains or lines available

Notes

Cre recombinase-mediated recombination between the loxP site downstream of this insertion and a second loxP site elsewhere in the homologous chromosome may be used to delete the genes between the loxP sites, placing the gene following the deletion immediately downstream of Hoxd11 with its cis-acting regulatory elements.

References

• Original: J:33879 van der Hoeven F, et al., Gene transpositions in the HoxD complex reveal a hierarchy of regulatory controls. Cell. 1996 Jun 28;85(7):1025-35
• All: 5 reference(s)