Tg(Spo11-cre)D5Mpel
Transgene Detail

Summary

• Symbol: Tg(Spo11-cre)D5Mpel
• Name: transgene insertion D5, Manuela Pellegrini
• MGI ID: MGI:4850038
• Synonyms: Spo11^Cre, Spo11-IRES-Cre
• Transgene: Tg(Spo11-cre)D5Mpel Location: unknown
• Alliance: Tg(Spo11-cre)D5Mpel page

Transgene origin

• Strain of Origin: BALB/c x C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Spo11-cre)D5Mpel expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Spo11 (MGI:1349669)

• Mutation details: The BAC RP23-20N4 containing the entire Spo11 locus (including 188kb of sequence upstream of the initiation codon and 57kb downstream of the stop codon) was engineered by homologous recombination to insert an IRES-cre-(Frt-flanked) neo fragment immediately downstream of the stop codon. The neo was subsequently removed by Flp in bacteria. The modified BAC was injected into the pronuclei of fertilized eggs. Two named lines, D5 and H9, were generated and studied further. (J:166888)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spo11 (mouse) Summary of all recombinase alleles driven by Spo11. Alliance mouse Spo11 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:166888 Pellegrini M, et al., Targeted JAM-C deletion in germ cells by Spo11-controlled Cre recombinase. J Cell Sci. 2011 Jan 1;124(Pt 1):91-9
• All: 4 reference(s)