Abl1^tm2.2Goff
Targeted Allele Detail

Summary

• Symbol: Abl1^tm2.2Goff
• Name: c-abl oncogene 1, non-receptor tyrosine kinase; targeted mutation 2.2, Stephen P Goff
• MGI ID: MGI:4850041
• Synonyms: Abl^delta
• Gene: Abl1 Location: Chr2:31578388-31694239 bp, + strand Genetic Position: Chr2, 21.86 cM, cytoband B
• Alliance: Abl1^tm2.2Goff page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166211
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted at the end of intron 4 and a neo cassette flanked by two loxP sites were inserted into the middle of intron 6. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. Excision of the fifth exon and sixth exon by Cre recombinase deletes part of the tyrosine kinase domain and fully inactivate the gene as determined by Western blot, creating a null allele. (J:166211)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abl1 Mutation: 93 strains or lines available

References

• Original: J:166211 Qiu Z, et al., Abl family tyrosine kinases are essential for basement membrane integrity and cortical lamination in the cerebellum. J Neurosci. 2010 Oct 27;30(43):14430-9
• All: 2 reference(s)