Grn^tm1Blrl
Targeted Allele Detail

Summary

• Symbol: Grn^tm1Blrl
• Name: granulin; targeted mutation 1, Blair R Leavitt
• MGI ID: MGI:4867226
• Synonyms: Grn^fleo, GrnKO
• Gene: Grn Location: Chr11:102321333-102327635 bp, + strand Genetic Position: Chr11, 66.29 cM
• Alliance: Grn^tm1Blrl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167031
• Parent Cell Line: mEMS128 (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 3. A cassette, which contains (5' to 3') a loxP site, FRT site, splice acceptor, IRES, beta-geo cassette, stop codon, polyadenylation sequence, and FRT site was inserted downstream of exon 4. The inserted sequence disrupts expression of endogenous Grn and instead expresses a lacZ. Immunohistochemistry and western blot analysis confirmed the lack of protein expression in the brain. (J:167031, J:182051)

Expression

In Mice Carrying this Mutation:

54 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grn Mutation: 48 strains or lines available

References

• Original: J:167031 Petkau TL, et al., Progranulin expression in the developing and adult murine brain. J Comp Neurol. 2010 Oct 1;518(19):3931-47
• All: 4 reference(s)