B3gnt5^tm2.1Big
Targeted Allele Detail

Summary

• Symbol: B3gnt5^tm2.1Big
• Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5; targeted mutation 2.1, Darell D Bigner
• MGI ID: MGI:4867403
• Gene: B3gnt5 Location: Chr16:19578958-19591503 bp, + strand Genetic Position: Chr16, 12.23 cM, cytoband B1
• Alliance: B3gnt5^tm2.1Big page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166763
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding region in exon 4 was flanked with loxP sites via homologous recombination. Cre mediated recombination removed the floxed region. RT-PCR confirmed the absence of expression in multiple tissues in fetal and adult mice. (J:166763)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any B3gnt5 Mutation: 24 strains or lines available

References

• Original: J:166763 Kuan CT, et al., Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase--a key enzyme in lacto-neolacto ganglioside synthesis. BMC Dev Biol. 2010;10:114
• All: 1 reference(s)