Mesp2^{tm16.1(Rbpj*)Ysa}
Targeted Allele Detail

Summary

• Symbol: Mesp2^{tm16.1(Rbpj*)Ysa}
• Name: mesoderm posterior 2; targeted mutation 16.1, Yumiko Saga
• MGI ID: MGI:4867475
• Synonyms: deltaneoMesp2-R218H, Mesp2^R218H
• Gene: Mesp2 Location: Chr7:79460475-79463179 bp, + strand Genetic Position: Chr7, 45.18 cM
• Alliance: Mesp2^{tm16.1(Rbpj*)Ysa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167045
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Dominant negative, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mesp2^{tm16.1(Rbpj*)Ysa} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Rbpj (MGI:96522)

• Mutation details: The entire gene was replaced with a mutated Rbpj cDNA coding for histidine rather than arginine at position 218, a floxed neo cassette, and a poly A tail. Cre mediated recombination removed the neo cassette. This allele expresses a dominant negative form of Rbpj. Whole mount in situ hybridization analysis confirmed the absence of Mesp2 expression in E11.5 homozygous embryos. (J:167045)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mesp2 Mutation: 30 strains or lines available

References

• Original: J:167045 Sasaki N, et al., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development. 2011 Jan;138(1):55-64
• All: 1 reference(s)