Kmt2b^tm2.1Afst
Targeted Allele Detail

Summary

• Symbol: Kmt2b^tm2.1Afst
• Name: lysine (K)-specific methyltransferase 2B; targeted mutation 2.1, A Francis Stewart
• MGI ID: MGI:4867487
• Synonyms: Mll2^tm2, Mll2^tm2afst
• Gene: Kmt2b Location: Chr7:30268283-30288151 bp, - strand Genetic Position: Chr7, 18.63 cM, cytoband A3
• Alliance: Kmt2b^tm2.1Afst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166778
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: An enhanced yellow fluorescence protein (eYFP) cassette was introduced in frame with the first ATG in exon 1, followed by a neo cassette flanked by two Frt sites. The neo cassette was removed by Flp-mediated recombination, resulting in a continuous reading frame from the initiating codon, through eYFP and the residual FRT to the second amino acid. No yellow fluorescent signal was detected in any tissue. Real-time PCR and Western blot analysis showed decreased expression in ovaries from homozygous females. (J:166778)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2b Mutation: 65 strains or lines available

References

• Original: J:166778 Andreu-Vieyra CV, et al., MLL2 is required in oocytes for bulk histone 3 lysine 4 trimethylation and transcriptional silencing. PLoS Biol. 2010;8(8)
• All: 1 reference(s)