Tg(DCX-cre/ERT2)2Wrst
Transgene Detail

Summary

• Symbol: Tg(DCX-cre/ERT2)2Wrst
• Name: transgene insertion 2, Wolfgang Wurst
• MGI ID: MGI:4867631
• Synonyms: DCX-CreERT2
• Transgene: Tg(DCX-cre/ERT2)2Wrst Location: unknown
• Alliance: Tg(DCX-cre/ERT2)2Wrst page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(DCX-cre/ERT2)2Wrst expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  DCX (1641)

  Dcx	(MGI:1277171)

• Mutation details: A fragment containing the cre/ERT2 sequence was inserted into a plasmid containing the human DCX promoter. A 7.7 kb fragment of the 3' UTR of DCX was generated by PCR and inserted into the fragment. The construct was injected into FVB fertilized oocytes. Two founders were obtained, with both displaying the expected recombination pattern with tamoxifen treatment after crossing to reporter mice. Southern blot analysis suggested only 1 copy of the transgene had been integrated into the genome. Since activity could be detected in line 1 with no tamoxifen treatment, only line 2 was propagated further. (J:167201)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	DCX (human) Summary of all recombinase alleles driven by DCX. Alliance human DCX gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:167201 Zhang J, et al., A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons. BMC Neurosci. 2010 Dec 31;11(1):158
• All: 6 reference(s)