Otx2^tm8.2Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm8.2Sia
• Name: orthodenticle homeobox 2; targeted mutation 8.2, Shinichi Aizawa
• MGI ID: MGI:4867634
• Synonyms: Otx2^{deltaFM1deltaFM2}
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm8.2Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166617
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The FM1 enhancer was replaced with a floxed neo cassette while the FM2 enhancer was replaced with an FRT-flanked puro cassette. Flp- and cre-mediated recombination remove the selection cassettes leaving a single loxP site in place of FM1 and a single FRT site in place of FM2. (J:166617)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:166617 Sakurai Y, et al., Otx2 and Otx1 protect diencephalon and mesencephalon from caudalization into metencephalon during early brain regionalization. Dev Biol. 2010 Nov 15;347(2):392-403
• All: 1 reference(s)