Spdef^tm1Shiv
Targeted Allele Detail

Summary

• Symbol: Spdef^tm1Shiv
• Name: SAM pointed domain containing ets transcription factor; targeted mutation 1, Ramesh Shivdasani
• MGI ID: MGI:4867866
• Gene: Spdef Location: Chr17:27933326-27947929 bp, - strand Genetic Position: Chr17, 14.59 cM, cytoband B1
• Alliance: Spdef^tm1Shiv page

Polypoid antral hyperplasia in Spdef^tm1Shiv/Spdef^tm1Shiv mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166876
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exons 2-5 were replaced with a PGK-neomycin resistance cassette. This removed the transactivation domain, the Pointed domain, and most of the Ets DNA binding domain. Absence of protein was confirmed by immunostaining. (J:166876)

Generation of the Spdef^tm1Shiv allele

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spdef Mutation: 15 strains or lines available

References

• Original: J:166876 Horst D, et al., Requirement of the epithelium-specific Ets transcription factor Spdef for mucous gland cell function in the gastric antrum. J Biol Chem. 2010 Nov 5;285(45):35047-55
• All: 1 reference(s)