Dtymk^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Dtymk^tm1(KOMP)Mbp
• Name: deoxythymidylate kinase; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4867962
• Gene: Dtymk Location: Chr1:93720298-93729656 bp, - strand Genetic Position: Chr1, 47.34 cM
• Alliance: Dtymk^tm1(KOMP)Mbp page
• IMPC: Dtymk gene page

Mutation origin

• Mutant Cell Lines: DEPD00545_4_A09, DEPD00545_4_A11, DEPD00545_4_B09, DEPD00545_4_B10, DEPD00545_4_B11, DEPD00545_4_B12, DEPD00545_4_C09, DEPD00545_4_C10, DEPD00545_4_C11, DEPD00545_4_C12, DEPD00545_4_D09, DEPD00545_4_D10, DEPD00545_4_D12, DEPD00545_4_E09, DEPD00545_4_E10, DEPD00545_4_E12, DEPD00545_4_F09, DEPD00545_4_F10, DEPD00545_4_F11, DEPD00545_4_F12, DEPD00545_4_G09, DEPD00545_4_G10, DEPD00545_4_G11, DEPD00545_4_H09, DEPD00545_4_H10, DEPD00545_4_H12
• Germline Transmission: Earliest citation of germline transmission: J:347587
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2595 starting at position 93726038 and ending at position 93728633 of Chromosome 1 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Dtymk Mutation: 22 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 2 reference(s)