Csgalnact1^tm1.1Migar
Targeted Allele Detail

Summary

• Symbol: Csgalnact1^tm1.1Migar
• Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1; targeted mutation 1.1, Michihiro Igarashi
• MGI ID: MGI:4868209
• Synonyms: T1KO
• Gene: Csgalnact1 Location: Chr8:68809433-69187798 bp, - strand Genetic Position: Chr8, 33.88 cM
• Alliance: Csgalnact1^tm1.1Migar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166870
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 7, and an additional loxP site was inserted downstream of exon 7. Cre-mediated recombination removed exon 7 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on E18.5 head extracts. (J:166870)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	23 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Csgalnact1 Mutation: 33 strains or lines available

References

• Original: J:166870 Watanabe Y, et al., Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development. Biochem J. 2010 Oct 25;432(1):47-55
• All: 8 reference(s)