Tg(Pax4-cre)1Dam
Transgene Detail

Summary

• Symbol: Tg(Pax4-cre)1Dam
• Name: transgene insertion 1, Douglas A Melton
• MGI ID: MGI:4868408
• Synonyms: Pax4-Cre
• Transgene: Tg(Pax4-cre)1Dam Location: unknown
• Alliance: Tg(Pax4-cre)1Dam page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Pax4-cre)1Dam expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pax4 (MGI:97488)

• Mutation details: The Pax4-cre transgene was designed with the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a tet-off cassette (EGFP/tTA). The transgene also contained a tetracycline-responsive element witha CMV minimal promoter (tetO-CMVmin) driving expression of a cre-recombinase. (J:119944)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pax4 (mouse) Summary of all recombinase alleles driven by Pax4. Comparative matrix view of recombinase activities Alliance mouse Pax4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

These Pax4-cre transgenic mice function only for cre recombinase expression in Pax4-expressing cells (no tet-Off or fluorescence function is reported). The transgene was microinjected into fertilized (C57BL/6 X CBA)F1 oocytes, and founder mice were bred to ICR outbred mice to establish a colony.

References

• Original: J:119944 Greenwood AL, et al., Notch signaling reveals developmental plasticity of Pax4(+) pancreatic endocrine progenitors and shunts them to a duct fate. Mech Dev. 2007 Feb;124(2):97-107
• All: 2 reference(s)