Prkab2^tm1.1Grst
Targeted Allele Detail

Summary

• Symbol: Prkab2^tm1.1Grst
• Name: protein kinase, AMP-activated, beta 2 non-catalytic subunit; targeted mutation 1.1, Gregory R Steinberg
• MGI ID: MGI:4868754
• Gene: Prkab2 Location: Chr3:97565527-97581128 bp, + strand Genetic Position: Chr3, 42.25 cM
• Alliance: Prkab2^tm1.1Grst page

Smaller Type IIA and IIB muscle fibers in Prkab2^tm1.1Grst/Prkab2^tm1.1Grst extensor digitorium longus muscles

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167339
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A loxP was inserted upstream of exon 2, and a floxed neo cassette was inserted downstream of exon 4. Cre-mediated recombination removed exons 2 through 4 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on heart and red vastus extracts. (J:167339)

Generation of the Prkab2^tm1Grst and Prkab2^tm1.1Grst alleles

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkab2 Mutation: 29 strains or lines available

References

• Original: J:167339 Steinberg GR, et al., Whole body deletion of AMP-activated protein kinase {beta}2 reduces muscle AMPK activity and exercise capacity. J Biol Chem. 2010 Nov 26;285(48):37198-209
• All: 7 reference(s)