Gt(ROSA)26Sor^{tm1.1(CAG-Fto)Rdc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-Fto)Rdc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Roger D Cox
• MGI ID: MGI:4879001
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-Fto)Rdc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166829
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-Fto)Rdc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fto (MGI:1347093)

• Mutation details: A construct containing a CAGGs promoter, a floxed neo stop cassette, an Fto cDNA, and an FRT flanked IRES-GFP cassette was inserted via homologous recombination. Cre mediated recombination removed the floxed neo-stop cassette. Heterozygous mice show about an 8 fold increase in protein expression in the pancreas. Homozygous mice show about an 11 fold increase in expression in skeletal muscle. (J:166829)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:166829 Church C, et al., Overexpression of Fto leads to increased food intake and results in obesity. Nat Genet. 2010 Dec;42(12):1086-92
• All: 3 reference(s)