Hectd3^{tm1(NCOM)Cmhd}
Targeted Allele Detail

Summary

• Symbol: Hectd3^{tm1(NCOM)Cmhd}
• Name: HECT domain E3 ubiquitin protein ligase 3; targeted mutation 1, Centre for Modeling Human Disease
• MGI ID: MGI:4880080
• Gene: Hectd3 Location: Chr4:116852514-116862474 bp, + strand Genetic Position: Chr4, 53.41 cM
• Alliance: Hectd3^{tm1(NCOM)Cmhd} page
• IMPC: Hectd3 gene page

Mutation origin

• Mutant Cell Line: N01346P1_C_60T_C5
• Germline Transmission: Earliest citation of germline transmission: J:211773
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_GOHANU
• Mutation details: The insertion of NorCOMM cassette pGOHANU created a deletion of size 3251bp between positions 116855090-116858341 of Chromosome 4 (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hectd3 Mutation: 42 strains or lines available

References

• Original: J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010
• All: 4 reference(s)