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Ostf1tm1(NCOM)Cmhd
Targeted Allele Detail
Summary
Symbol: Ostf1tm1(NCOM)Cmhd
Name: osteoclast stimulating factor 1; targeted mutation 1, Centre for Modeling Human Disease
MGI ID: MGI:4880102
Synonyms: Ostf1lacZ
Gene: Ostf1  Location: Chr19:18557728-18609177 bp, - strand  Genetic Position: Chr19, 13.17 cM
Alliance: Ostf1tm1(NCOM)Cmhd page
IMPC: Ostf1 gene page
Mutation
origin
Mutant Cell Lines:  N01551P0_C_27T_E12, N01551P0_C_29T_A2, N01551P0_C_29T_A5, N01551P0_C_29T_B2, N01551P0_C_29T_D1, N01551P0_C_29T_D2, N01551P0_C_29T_E6, N01551P0_C_29T_G3, N01551P0_C_29T_H2, N01551P0_C_29T_H3, N01551P0_C_29T_H6
Germline Transmission:  Earliest citation of germline transmission: J:249839
Parent Cell Line:  C2 (Tcp) (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: NorCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_NTARU-0
 
Mutation detailsThe insertion of NorCOMM cassette pNTARU created a deletion of size 3275bp between positions 18570945-18574220 of Chromosome 19 (Build GRCm39). This deletion results in the removal of functionally critical exons 3 and 4. Western blots confirm the absence of protein expression from this allele (other than the beta-galactosidase reporter). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 148 assay results
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ostf1 Mutation:  97 strains or lines available
References
Original:  J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory