Itpr3^{tm1(NCOM)Mfgc}
Targeted Allele Detail

Summary

• Symbol: Itpr3^{tm1(NCOM)Mfgc}
• Name: inositol 1,4,5-triphosphate receptor 3; targeted mutation 1, Mammalian Functional Genomics Centre
• MGI ID: MGI:4880235
• Gene: Itpr3 Location: Chr17:27276278-27341197 bp, + strand Genetic Position: Chr17, 13.68 cM
• Alliance: Itpr3^{tm1(NCOM)Mfgc} page
• IMPC: Itpr3 gene page

Mutation origin

• Mutant Cell Lines: N01293P1_C_239W_B6, N01293P1_C_239W_C5, N01293P1_C_239W_C6, N01293P1_C_239W_D6, N01293P1_C_239W_E5, N01293P1_C_239W_F5, N01293P1_C_239W_F6, N01293P1_C_239W_H5
• Germline Transmission: Earliest citation of germline transmission: J:201643
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_GOHANU
• Mutation details: The insertion of NorCOMM cassette pGOHANU created a deletion of size 1030bp between positions 27291602-27292632 of Chromosome 17 (Build GRCm39). This deletion results in the removal of functionally critical exon 2. The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itpr3 Mutation: 176 strains or lines available

Notes

Cell line N01293P1_C_239W_C5 was successfully used to make chimeric mice. Germline transmission was accomplished. J:201643

References

• Original: J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010
• All: 3 reference(s)