Dhrs3^{tm1(NCOM)Mfgc}
Targeted Allele Detail

Summary

• Symbol: Dhrs3^{tm1(NCOM)Mfgc}
• Name: dehydrogenase/reductase 3; targeted mutation 1, Mammalian Functional Genomics Centre
• MGI ID: MGI:4880251
• Gene: Dhrs3 Location: Chr4:144619397-144654779 bp, + strand Genetic Position: Chr4, 78.14 cM
• Alliance: Dhrs3^{tm1(NCOM)Mfgc} page
• IMPC: Dhrs3 gene page

Cleft palate, small eyes, and unfused eyelids in E18.5 Dhrs3^{tm1(NCOM)Mfgc}/Dhrs3^{tm1(NCOM)Mfgc} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: N01359P1_C_97W_A2, N01359P1_C_97W_B2, N01359P1_C_97W_C1, N01359P1_C_97W_D2, N01359P1_C_97W_D3, N01359P1_C_97W_E2, N01359P1_C_97W_G1
• Germline Transmission: Earliest citation of germline transmission: J:214109
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_GOHANU Mutation details: The insertion of NorCOMM cassette pGOHANU created a deletion of size 6002bp between positions 144644767-144650769 of Chromosome 4 (Build GRCm39). This deletion results in the removal of functionally critical exons 2-5. The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). Western blot confirmed absence of protein in E14.5 embryonic fibroblasts. (J:165964, J:214109)

Generation of the Dhrs3^tm1(NCOM)Mfgc allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dhrs3 Mutation: 25 strains or lines available

Notes

Cell lines N01359P1_C_97W_B2 and N01359P1_C_97W_G1 were successfully used to make chimeric mice. Germline transmission was accomplished. J:214109.

References

• Original: J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010
• All: 2 reference(s)