Prkag1^tm1.2Mfor
Targeted Allele Detail

Summary

• Symbol: Prkag1^tm1.2Mfor
• Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit; targeted mutation 1.2, Marc Foretz
• MGI ID: MGI:4880680
• Gene: Prkag1 Location: Chr15:98710678-98729354 bp, - strand Genetic Position: Chr15, 54.73 cM
• Alliance: Prkag1^tm1.2Mfor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167779
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked hygro cassette with a 3' loxP site was inserted upstream of exon 3, and an additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the hygro cassette and cre-mediated recombination removed exons 3 through 7 floxed. The absence of protein expression was confirmed in liver, muscle, white adipose tissue, brown adipose tissue, kidney, and spleen extracts. (J:167779)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkag1 Mutation: 29 strains or lines available

References

• Original: J:167779 Foretz M, et al., The AMPKgamma1 subunit plays an essential role in erythrocyte membrane elasticity, and its genetic inactivation induces splenomegaly and anemia. FASEB J. 2011 Jan;25(1):337-47
• All: 3 reference(s)