Gns^{tm1e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gns^{tm1e(EUCOMM)Hmgu}
• Name: glucosamine (N-acetyl)-6-sulfatase; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4881048
• Gene: Gns Location: Chr10:121200995-121233154 bp, + strand Genetic Position: Chr10, 69.62 cM
• Alliance: Gns^{tm1e(EUCOMM)Hmgu} page
• IMPC: Gns gene page

Mutation origin

• Mutant Cell Lines: HEPD0697_8_A07, HEPD0697_8_B06, HEPD0697_8_E07
• Germline Transmission: Earliest citation of germline transmission: J:242250
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 121213421 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Gns Mutation: 26 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)