Dagla^tm1.1Kano
Targeted Allele Detail

Summary

• Symbol: Dagla^tm1.1Kano
• Name: diacylglycerol lipase, alpha; targeted mutation 1.1, Masanobu Kano
• MGI ID: MGI:4881474
• Synonyms: alpha-KO, DGLalpha KO
• Gene: Dagla Location: Chr19:10222629-10282241 bp, - strand Genetic Position: Chr19, 6.55 cM
• Alliance: Dagla^tm1.1Kano page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167891
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3, and an additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the neo cassette and exons 3 and 4. The absence of protein expression was confirmed by immunohistochemistry on the brain. (J:167891)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dagla Mutation: 62 strains or lines available

References

• Original: J:167891 Tanimura A, et al., The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission. Neuron. 2010 Feb 11;65(3):320-7
• All: 10 reference(s)