Daglb^tm1.1Kano
Targeted Allele Detail

Summary

• Symbol: Daglb^tm1.1Kano
• Name: diacylglycerol lipase, beta; targeted mutation 1.1, Masanobu Kano
• MGI ID: MGI:4881524
• Synonyms: beta-KO, DGLbeta KO
• Gene: Daglb Location: Chr5:143450239-143490197 bp, + strand Genetic Position: Chr5, 82.19 cM
• Alliance: Daglb^tm1.1Kano page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167891
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette with a 5' loxP site was inserted upstream of exon 10, and an additional loxP site was inserted downstream of exon 11. Cre-mediated recombination removed the neo cassette and exons 10 and 11. The absence of transcript expression was coonfirmed by western blot analysis on forebrain and cerebellum extracts. (J:167891)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Daglb Mutation: 24 strains or lines available

References

• Original: J:167891 Tanimura A, et al., The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission. Neuron. 2010 Feb 11;65(3):320-7
• All: 2 reference(s)