Mylk3^tm1Jtst
Targeted Allele Detail

Summary

• Symbol: Mylk3^tm1Jtst
• Name: myosin light chain kinase 3; targeted mutation 1, James Stull
• MGI ID: MGI:4881762
• Synonyms: cMLCK^neo
• Gene: Mylk3 Location: Chr8:86050933-86112969 bp, - strand Genetic Position: Chr8, 41.61 cM
• Alliance: Mylk3^tm1Jtst page

Mylk3^tm1Jtst/Mylk3⁺ and Mylk3^tm1Jtst/Mylk3^tm1Jtst mice exhibit hypertrophic hearts

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167590
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 4, and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. The absence of protein expression was confirmed by western blot analysis on ventricular and atrial extracts. (J:167590)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mylk3 Mutation: 42 strains or lines available

References

• Original: J:167590 Ding P, et al., Cardiac myosin light chain kinase is necessary for myosin regulatory light chain phosphorylation and cardiac performance in vivo. J Biol Chem. 2010 Dec 24;285(52):40819-29
• All: 1 reference(s)