Fam3b^tm1Bkht
Targeted Allele Detail

Summary

• Symbol: Fam3b^tm1Bkht
• Name: FAM3 metabolism regulating signaling molecule B; targeted mutation 1, Brant Burkhardt
• MGI ID: MGI:4882127
• Synonyms: PANDER^-
• Gene: Fam3b Location: Chr16:97272165-97306136 bp, - strand Genetic Position: Chr16, 57.47 cM
• Alliance: Fam3b^tm1Bkht page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167823
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2 were replaced with a neomycin selection cassette in reverse orientation. This removed the transcriptional and translational start sites and the first 54 amino acids including the secretion signal peptide and helix A. mRNA was not detected in pancreatic islets, small intestine, liver, spleen, kidney, and brain by RT-PCR. Western blot analysis of pancratic islets demonstrated the absence of full length protein. (J:167823)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fam3b Mutation: 22 strains or lines available

References

• Original: J:167823 Robert-Cooperman CE, et al., Targeted disruption of pancreatic-derived factor (PANDER, FAM3B) impairs pancreatic beta-cell function. Diabetes. 2010 Sep;59(9):2209-18
• All: 3 reference(s)