Tg(Pmch-cre)1Lowl
Transgene Detail

Summary

• Symbol: Tg(Pmch-cre)1Lowl
• Name: transgene insertion 1, Bradford B Lowell
• MGI ID: MGI:4887235
• Synonyms: MCH-Cre
• Transgene: Tg(Pmch-cre)1Lowl Location: unknown
• Alliance: Tg(Pmch-cre)1Lowl page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Pmch-cre)1Lowl expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Pmch

• Mutation details: Homologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further. (J:167906)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pmch (species not specified) Summary of all recombinase alleles driven by Pmch. Comparative matrix view of recombinase activities

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:167906 Kong D, et al., Glucose stimulation of hypothalamic MCH neurons involves K(ATP) channels, is modulated by UCP2, and regulates peripheral glucose homeostasis. Cell Metab. 2010 Nov 3;12(5):545-52
• All: 30 reference(s)