Ryr1^tm1Alle
Targeted Allele Detail

Summary

• Symbol: Ryr1^tm1Alle
• Name: ryanodine receptor 1, skeletal muscle; targeted mutation 1, Paul D Allen
• MGI ID: MGI:4887253
• Synonyms: Ry1⁵³
• Gene: Ryr1 Location: Chr7:28702765-28824599 bp, - strand Genetic Position: Chr7, 16.94 cM, cytoband A2-B3
• Alliance: Ryr1^tm1Alle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111187
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted in the KPN site at nt840 in exon10 to disrupt gene expression. (personal communication) Absence of protein expression was demonstrated by SDS-PAGE. (J:111187)

Expression

In Mice Carrying this Mutation:

6 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ryr1 Mutation: 214 strains or lines available

References

• Original: J:111187 Buck ED, et al., Dyspedic mouse skeletal muscle expresses major elements of the triadic junction but lacks detectable ryanodine receptor protein and function. J Biol Chem. 1997 Mar 14;272(11):7360-7
• All: 13 reference(s)