Mbd3^tm2Bh
Targeted Allele Detail

Summary

• Symbol: Mbd3^tm2Bh
• Name: methyl-CpG binding domain protein 3; targeted mutation 2, Brian Hendrich
• MGI ID: MGI:4887391
• Synonyms: Mbd3^2lox, mbd3^f, Mbd3^Flox, mdb3^Ex1-Flox
• Gene: Mbd3 Location: Chr10:80228373-80235365 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Mbd3^tm2Bh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167984
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: This allele, in which exon 1 is flanked by loxP sites, was generated in embryonic stem (ES) cells heterozygous for Mbd3^tm1Bh using a negative selection approach. First, a loxP-flanked hygromycin/thymidine kinase (hyg/tk) cassette was introduced into the wild-type allele upstream of exon 1. The ES cells were re-targeted with a vector having loxP sites flanking exon 1; two rounds of exposure to 6-thioguanine selected for cells in which the hyg/tk-tagged exon 1 had been replaced by the floxed exon. Immunoblot analysis of nuclear extracts of ES cells heterozygous for this floxed allele and the null Mbd3^tm1Bh allele using antibody to MBD3 demonstrated expression of both normal protein isoforms, one of which (referred to as Mbda) contains the entire methyl-CpG-binding domain (MBD) and the other (Mbdb) lacking the N-terminal half of the MBD, encoded by exon 1. Following Cre recombinase-mediated excision of exon 1, neither isoform is detected. (J:106974, J:167984)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mbd3 Mutation: 58 strains or lines available

References

• Original: J:106974 Kaji K, et al., The NuRD component Mbd3 is required for pluripotency of embryonic stem cells. Nat Cell Biol. 2006 Mar;8(3):285-92
• All: 7 reference(s)