Gnas^tm3Jop
Targeted Allele Detail

Summary

• Symbol: Gnas^tm3Jop
• Name: GNAS complex locus; targeted mutation 3, Jo Peters
• MGI ID: MGI:4887403
• Synonyms: Ex1A-T-CON
• Gene: Gnas Location: Chr2:174126113-174188537 bp, + strand Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
• Alliance: Gnas^tm3Jop page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182868
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A rabbit beta-globin polyadenylation cassette was inserted into the end of the exon 1A in reverse orientation so that it does not truncate the exon 1A transcript. A loxP flanked neomycin cre cassette was inserted immediately downstream as well. RT-PCR analysis indicated reduced mRNA levels were produced. Northern blot analysis did not detect mRNA. (J:182868)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnas Mutation: 53 strains or lines available

Notes

ES cell = MAC3.

References

• Original: J:182868 Eaton SA, et al., New mutations at the imprinted Gnas cluster show gene dosage effects of Gsalpha in postnatal growth and implicate XLalphas in bone and fat metabolism but not in suckling. Mol Cell Biol. 2012 Mar;32(5):1017-29
• All: 1 reference(s)