Sp3^tm2.1Sus
Targeted Allele Detail

Summary

• Symbol: Sp3^tm2.1Sus
• Name: trans-acting transcription factor 3; targeted mutation 2.1, Guntram Suske
• MGI ID: MGI:4887404
• Synonyms: Sp3^ki
• Gene: Sp3 Location: Chr2:72766774-72810790 bp, - strand Genetic Position: Chr2, 43.35 cM, cytoband C3
• Alliance: Sp3^tm2.1Sus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167521
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Not Applicable) Mutations: Insertion, Nucleotide substitutions Mutation details: Exon 5 was replaced with one in which nucleotide substitutions result in the amino acid substitution of glutamic acid for aspartic acid at position 553 (D553E). This allele is expected to produce a SUMOylation-deficient protein. A floxed lacZ-neo cassette inserted downstream of the modified exon 5 was removed by cre-mediated recombination. (J:167521)

Generation of the Sp3^tm2.1Sus allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sp3 Mutation: 64 strains or lines available

References

• Original: J:167521 Stielow B, et al., Epigenetic silencing of spermatocyte-specific and neuronal genes by SUMO modification of the transcription factor Sp3. PLoS Genet. 2010;6(11):e1001203
• All: 1 reference(s)