Usp21^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Usp21^{tm1a(EUCOMM)Wtsi}
• Name: ubiquitin specific peptidase 21; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4887724
• Gene: Usp21 Location: Chr1:171109523-171115534 bp, - strand Genetic Position: Chr1, 79.3 cM
• Alliance: Usp21^{tm1a(EUCOMM)Wtsi} page
• IMPC: Usp21 gene page

Mutation origin

• Mutant Cell Lines: EPD0716_10_A03, EPD0716_10_A04, EPD0716_10_A08, EPD0716_10_A10, EPD0716_10_B01, EPD0716_10_B03, EPD0716_10_B05, EPD0716_10_C01, EPD0716_10_C06, EPD0716_10_D04, EPD0716_10_D05, EPD0716_10_D06, EPD0716_10_E05, EPD0716_10_E12, EPD0716_10_F02, EPD0716_10_F11, EPD0716_10_G07, EPD0716_10_G09, EPD0716_10_H06, EPD0716_10_H09, EPD0716_10_H12, EPD0790_1_A08, EPD0790_1_B05, EPD0790_1_B06, EPD0790_1_B11, EPD0790_1_C03, EPD0790_1_D02, EPD0790_1_D11, EPD0790_1_E02, EPD0790_1_E04, EPD0790_1_E10, EPD0790_1_F01, EPD0790_1_F12, EPD0790_1_G01, EPD0790_1_G05, EPD0790_1_H02, EPD0790_1_H04
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 171111927 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 171113776. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Usp21 Mutation: 26 strains or lines available

Notes

Cell line EPD0716_10_H09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:220984

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 8 reference(s)