Myh14^tm1Rsad
Targeted Allele Detail

Summary

• Symbol: Myh14^tm1Rsad
• Name: myosin, heavy polypeptide 14; targeted mutation 1, Robert S Adelstein
• MGI ID: MGI:4887985
• Synonyms: C^-
• Gene: Myh14 Location: Chr7:44255227-44320267 bp, - strand Genetic Position: Chr7, 28.85 cM, cytoband B2
• Alliance: Myh14^tm1Rsad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168304
• Parent Cell Line: CMT-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.3 kb fragment including the 157 bp exon 3 was replaced by a loxP-flanked neomycin resistance cassette via homologous recombination. Deletion of exon 3 was confirmed by Southern blot. Immunoblot analysis from lungs confirmed the absence of protein. (J:168304)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myh14 Mutation: 82 strains or lines available

References

• Original: J:168304 Ma X, et al., Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis. Mol Biol Cell. 2010 Nov;21(22):3952-62
• All: 4 reference(s)