Atf4^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Atf4^tm1(KOMP)Mbp
• Name: activating transcription factor 4; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4888784
• Gene: Atf4 Location: Chr15:80139385-80141742 bp, + strand Genetic Position: Chr15, 37.85 cM
• Alliance: Atf4^tm1(KOMP)Mbp page
• IMPC: Atf4 gene page

Mutation origin

• Mutant Cell Lines: DEPD00549_2_A06, DEPD00549_2_A07, DEPD00549_2_A08, DEPD00549_2_B06, DEPD00549_2_B07, DEPD00549_2_C05, DEPD00549_2_C06, DEPD00549_2_C07, DEPD00549_2_D06, DEPD00549_2_E05, DEPD00549_2_E07, DEPD00549_2_E08, DEPD00549_2_F06, DEPD00549_2_F08, DEPD00549_2_G06, DEPD00549_2_H05, DEPD00549_2_H06, DEPD00549_2_H08
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 957 starting at position 80140807 and ending at position 80141764 of Chromosome 15 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Atf4 Mutation: 22 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 4 reference(s)