Gnao1^tm2Lbi
Targeted Allele Detail

Summary

• Symbol: Gnao1^tm2Lbi
• Name: guanine nucleotide binding protein, alpha O; targeted mutation 2, Lutz Birnbaumer
• MGI ID: MGI:4889046
• Synonyms: Go1alpha^-
• Gene: Gnao1 Location: Chr8:94537213-94696016 bp, + strand Genetic Position: Chr8, 45.94 cM
• Alliance: Gnao1^tm2Lbi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168245
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Insertion Mutation details: An IRES-beta galactosidase and PGK-neomycin cassette was inserted in exon 7.1 at the aspartic acid position 262. Isoform Go1-alpha was disrupted while beta galactosidase was placed under the control of the Gnao1 promoter. Absence of the protein product from brain and pancreatic islets was confirmed immunologically. (J:168245)

Generation of the Gnao1^tm2Lbi allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnao1 Mutation: 29 strains or lines available

References

• Original: J:168245 Wang Y, et al., Augmented glucose-induced insulin release in mice lacking Go2, but not Go1 or Gi proteins. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1693-8
• All: 1 reference(s)