Gpa33^{tm1(GNAS)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gpa33^{tm1(GNAS)Wtsi}
• Name: glycoprotein A33 transmembrane; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4889205
• Synonyms: Gpa33^{tm1(GnasR201C)Wtsi}
• Gene: Gpa33 Location: Chr1:165957807-165994079 bp, + strand Genetic Position: Chr1, 73.49 cM
• Alliance: Gpa33^{tm1(GNAS)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168378
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gpa33^{tm1(GNAS)Wtsi} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GNAS (2778)

  Gnas	(MGI:95777)

• Mutation details: A cassette containing, a floxed IRES-neo/STOP cassette followed by an IRES and human GNAS with nucleotide substitutions that result in the amino acid subsitution of cysteine for arginine at position 201 (R201C), was inserted downstream of the coding sequence in exon 7. This mutation produces a constitutively active protein. (J:168378)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpa33 Mutation: 138 strains or lines available

References

• Original: J:168378 Wilson CH, et al., The activating mutation R201C in GNAS promotes intestinal tumourigenesis in Apc(Min/+) mice through activation of Wnt and ERK1/2 MAPK pathways. Oncogene. 2010 Aug 12;29(32):4567-75
• All: 1 reference(s)