Mecp2^tm3.1Bird
Targeted Allele Detail

Summary

• Symbol: Mecp2^tm3.1Bird
• Name: methyl CpG binding protein 2; targeted mutation 3.1, Adrian Bird
• MGI ID: MGI:4938309
• Synonyms: MECP2-EGFP
• Gene: Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
• Alliance: Mecp2^tm3.1Bird page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168822
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to insert an enhanced green fluorescent protein sequence (EGFP) sequence (preceded by sequence for linker peptide CKDPPVAT) and a loxP-flanked neomycin (neo) resistance sequence-STOP cassette in the 3' UTR. Subsequent cre-mediated recombination removed the floxed neo-STOP cassette. This allele expresses a chimeric protein with the EGFP peptide linked to the C-terminus of the endogenous peptide. (J:168822)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mecp2 Mutation: 41 strains or lines available

References

• Original: J:168822 Bird A, Direct Data Submission 2011/02/25. MGI Direct Data Submission. 2011
• All: 14 reference(s)