Mbtd1^tm1Homy
Targeted Allele Detail

Summary

• Symbol: Mbtd1^tm1Homy
• Name: mbt domain containing 1; targeted mutation 1, Hiroaki Honda
• MGI ID: MGI:4940055
• Synonyms: Hemp^-
• Gene: Mbtd1 Location: Chr11:93776678-93837811 bp, + strand Genetic Position: Chr11, 58.86 cM
• Alliance: Mbtd1^tm1Homy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169112
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 6, 7, and 8 were replaced with an IRES GFP and a floxed neomycin resistance gene. The selection cassette was subsequently removed by cre mediated recombination. This removes the four "malignant brain tumor" domains. Western blot analysis demonstrated that no protein product was produced. (J:169112)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mbtd1 Mutation: 86 strains or lines available

References

• Original: J:169112 Honda H, et al., Hemp, an mbt domain-containing protein, plays essential roles in hematopoietic stem cell function and skeletal formation. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2468-73
• All: 1 reference(s)