Tg(Thy1-MAPT*)30Schd
Transgene Detail

Summary

• Symbol: Tg(Thy1-MAPT*)30Schd
• Name: transgene insertion 30, Katharina Schindowski
• MGI ID: MGI:4940070
• Synonyms: Tg30, THY-Tau30
• Transgene: Tg(Thy1-MAPT*)30Schd Location: unknown
• Alliance: Tg(Thy1-MAPT*)30Schd page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-MAPT*)30Schd expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAPT (4137)

  Mapt	(MGI:97180)

• Mutation details: cDNA of the 412-amino acid 4-repeat isoform of the human MAPT gene was reamplified and modified by PCR-based site directed mutagenesis to introduce a Kozak-sequence and the mutations G272V and P301S (numbering according to longest human isoform). The cDNA was inserted into a Thy1.2 expression vector and the construct was microinjected into a C57BL/6 x CBA background. 5-10 copies of the transgene are present in this line. Line 30 animals display high levels of human tau in the spinal cord. In the brain, high tau protein levels are observed in the hippocampus with low levels found in the cortex. (J:111982)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:111982 Schindowski K, et al., Alzheimer's disease-like tau neuropathology leads to memory deficits and loss of functional synapses in a novel mutated tau transgenic mouse without any motor deficits. Am J Pathol. 2006 Aug;169(2):599-616
• All: 8 reference(s)