Gt(ROSA)26Sor^{tm1(Pim1-E2F1)Rebr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Pim1-E2F1)Rebr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rene Bernards
• MGI ID: MGI:4940857
• Synonyms: R26-betageo-E2F1, R26E2F1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Pim1-E2F1)Rebr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83303
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Pim1-E2F1)Rebr} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  E2F1 (1869)

  E2f1	(MGI:101941)

• Mutation details: A human E2F1 cDNA was cloned downstream of a murine Pim1 promoter with a duplicated Ig heavy chain enhancer sequence. This construct was inserted downstream of a floxed beta-geo cassette and upstream of an IRES-PLAP cassette. Cre-mediated recombination of the STOP cassette is required for expression of the construct. (J:83303)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:83303 Scheijen B, et al., Constitutive E2F1 overexpression delays endochondral bone formation by inhibiting chondrocyte differentiation. Mol Cell Biol. 2003 May;23(10):3656-68
• All: 4 reference(s)