Tg(Slc2a4-cre)546Dac
Transgene Detail

Summary

• Symbol: Tg(Slc2a4-cre)546Dac
• Name: transgene insertion 546, Domenico Accili
• MGI ID: MGI:4941207
• Synonyms: GLUT4-Cre
• Transgene: Tg(Slc2a4-cre)546Dac Location: unknown
• Alliance: Tg(Slc2a4-cre)546Dac page

Transgene origin

• Strain of Origin: C57BL/6 x FVB

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Slc2a4-cre)546Dac expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Slc2a4

• Mutation details: A 2.4 kb construct containing the Slc2a4 (GLUT4) promoter driving expression of cre recombinase was injected into C57BL/6 x FVB fertilized eggs. Two founders were produced, 535 and 546, and characterized for transmission and recombination. Line 546 showed autosomal transmission and line 535 showed X-linked heritability. (Both lines underwent germ line recombination when transmitted through the dam. When transmitted through the sire, both transgenes were liable to stochastic em- bryonic activation, leading to generation of animals with varying degrees of chimerism.) Line 546 recapitulated endogenous Slc2a4 expression, exhibiting recombination in skeletal muscle, epididymal and subcutaneous white adipose tissue, brown adipose tissue, heart, and kidney. However, recombinase activity was observed ectopically in the ovarian corpora lutea, myometrium, nuclear layers of the retina, acinar cells in the exocrine pancreas, and salivary gland. Line 546 was studied in greater detail. (J:169404)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc2a4 (species not specified) Summary of all recombinase alleles driven by Slc2a4.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:169404 Lin HV, et al., Diabetes in mice with selective impairment of insulin action in glut4-expressing tissues. Diabetes. 2011 Mar;60(3):700-9
• All: 6 reference(s)