Nos1^{tm1.1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Nos1^{tm1.1(cre/ERT2)Zjh}
• Name: nitric oxide synthase 1, neuronal; targeted mutation 1.1, Z Josh Huang
• MGI ID: MGI:4941223
• Synonyms: nNOS-CreER-KI, nNOS-CreER-KI/KO, nNOS-CreERT2-KI, Nos1-CreER-KI, Nos1-CreERT2-KI
• Gene: Nos1 Location: Chr5:118004904-118096905 bp, + strand Genetic Position: Chr5, 57.29 cM, cytoband F
• Alliance: Nos1^{tm1.1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nos1^{tm1.1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nos1 (MGI:97360)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the neuronal nitric oxide synthase 1 locus (Nos1). This construct was electroporated into C57BL/6;129S-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S mice to originate the colony. Mutant mice were bred with Actin-FLPe mice to remove the neo selection cassette and the FLPe transgene was subsequently bred out of the line. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nos1 (mouse) Summary of all recombinase alleles driven by Nos1. Comparative matrix view of recombinase activities Alliance mouse Nos1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nos1 Mutation: 84 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 47 reference(s)