Gja5^{tm2(cre/ERT2,RFP)Lumi}
Targeted Allele Detail

Summary

• Symbol: Gja5^{tm2(cre/ERT2,RFP)Lumi}
• Name: gap junction protein, alpha 5; targeted mutation 2, Lucile Miquerol
• MGI ID: MGI:4941829
• Synonyms: Cx40-CreERT2
• Gene: Gja5 Location: Chr3:96939718-96960950 bp, + strand Genetic Position: Chr3, 42.04 cM
• Alliance: Gja5^{tm2(cre/ERT2,RFP)Lumi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169695
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Gja5^{tm2(cre/ERT2,RFP)Lumi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gja5 (MGI:95716)

• Mutation details: A cre/ERT2 cassette followed by an IRES::mRFP1 (monomeric RFP) sequence along with a PGK-neo cassette was introduced at the ATG site in exon 2 of the Gja5 locus, replacing the exon 2 sequence. (J:169695)

Generation of the Gja5^{tm2(cre/ERT2,RFP)Lumi} allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gja5 (mouse) Summary of all recombinase alleles driven by Gja5. Comparative matrix view of recombinase activities Alliance mouse Gja5 gene page

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja5 Mutation: 19 strains or lines available

References

• Original: J:169695 Beyer S, et al., Inducible Cx40-Cre expression in the cardiac conduction system and arterial endothelial cells. Genesis. 2011 Feb;49(2):83-91
• All: 10 reference(s)