Usp5^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Usp5^{tm1a(EUCOMM)Hmgu}
• Name: ubiquitin specific peptidase 5 (isopeptidase T); targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4942058
• Gene: Usp5 Location: Chr6:124791982-124806404 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Usp5^{tm1a(EUCOMM)Hmgu} page
• IMPC: Usp5 gene page

Mutation origin

• Mutant Cell Lines: HEPD0714_4_A08, HEPD0714_4_B06, HEPD0714_4_B08, HEPD0714_4_C05, HEPD0714_4_C06, HEPD0714_4_C07, HEPD0714_4_D05, HEPD0714_4_E05, HEPD0714_4_E07, HEPD0714_4_F06, HEPD0714_4_F07, HEPD0714_4_G05, HEPD0714_4_G06, HEPD0714_4_G07, HEPD0714_4_H06, HEPD0714_4_H08
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 124800878 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 124802574. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Usp5^tm1a(EUCOMM)Hmgu/Usp5^tm1a(EUCOMM)Hmgu	C57BL/6N-Usp5^tm1a(EUCOMM)Hmgu/Wtsi	HEPD0714_4_B06
ht2	Usp5^tm1a(EUCOMM)Hmgu/Usp5^+	C57BL/6N-Usp5^tm1a(EUCOMM)Hmgu/Wtsi	HEPD0714_4_B06

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms
Source:	IMPC - WTSI	IMPC - WTSI
hematopoietic system		√
decreased leukocyte cell number		√
immune system		√
decreased leukocyte cell number		√
mortality/aging	√
preweaning lethality, complete penetrance	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Usp5 Mutation: 51 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 4 reference(s)